VCAM1 anticorps (CF®740)

N° du produit ABIN7661830

Cet anticorps Souris Monoclonal détecte spécifiquement VCAM1 dans FACS, WB, IHC, IP et IHC (fp). Il présente une réactivité envers Humain.

Aperçu rapide pour VCAM1 anticorps (CF®740) (ABIN7661830)

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VCAM1 est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 1-4C3

Détail du produit anti-VCAM1 anticorps (CF®740)

Fonction: CD106 / VCAM1(1.4C3), CF740 conjugate

Attributs du produit: CD106 (also known as vascular cell adhesion molecule-1, or VCAM-1) is a member of the Ig superfamily of adhesion molecules. It is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte beta-1 integrin VLA-4 and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Stimulated human umbilical vein endothelial cells (HUVEC)

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Human placenta or tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur VCAM1

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Autre désignation: CD106

Sujet:

Synonyms: CD106, VCAM-1, Vascular Cell Adhesion Molecule 1, VCAM1, INCAM-100

Gene Symbol: VCAM1

Tissue Expression: Endothelial cells

Poids moléculaire: ~110 kDa

ID gène: 7412

UniProt: P19320

Pathways: Carbohydrate Homeostasis